pTARGEX-ER
(Plasmid #242449)

• Purpose: (Empty Backbone) Targets high levels of recombinant protein for retention in ER. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
• Depositing Lab: Allyson MacLean
• Publication: Chartrand-Pleau et al Front. Synth. Biol. 3:1619871

Backbone

• Vector backbone: pTARGEX
• Backbone manufacturer: uOttawa iGEM team 2024
• Backbone size (bp): 11636
• Modifications to backbone: Addition of N-terminal signal peptide derived from the Nicotiana tabacum pathogenesis-related protein 1b (PR1b) and a canonical C-terminal ER-retrieval motif (KDEL)
• Vector type: Plant Expression
• Promoter: double 35S
• Tags / Fusion Proteins:
  • Signal peptide derived from the Nicotiana tabacum pathogenesis-related protein 1b (N terminal on backbone)
  • ER-retrieval motif (KDEL) (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Clone and maintain in E. coli. For expression in planta, transform into suitable Agrobacterium tumefaciens strain and grow at 28C.
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry