MBP-GFP-RGG
(Plasmid
#242453)
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PurposeExpresses the MBP-GFP-RGG fusion protein.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242453 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET
- Backbone size w/o insert (bp) 5210
- Total vector size (bp) 7673
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMBP-GFP-RGG
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SpeciesC. elegans (nematode)
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Insert Size (bp)2463
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GenBank IDNC_003281.10
- Promoter T7
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Tags
/ Fusion Proteins
- MBP (N terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MBP-GFP-RGG was a gift from Benjamin Schuster (Addgene plasmid # 242453 ; http://n2t.net/addgene:242453 ; RRID:Addgene_242453) -
For your References section:
Amphiphilic proteins coassemble into multiphasic condensates and act as biomolecular surfactants. Kelley FM, Favetta B, Regy RM, Mittal J, Schuster BS. Proc Natl Acad Sci U S A. 2021 Dec 21;118(51):e2109967118. doi: 10.1073/pnas.2109967118. 10.1073/pnas.2109967118 PubMed 34916288