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pLV-puro-EF1A-3XFLAG-hASB8_ANK3-4
(Plasmid #242468)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242468 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    VectorBuilder pLV
  • Backbone manufacturer
    VectorBuilder
  • Backbone size w/o insert (bp) 8495
  • Total vector size (bp) 9431
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ASB8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    936
  • Mutation
    ANK3-4 = R87A - E95A - K96A - W124G - K128G
  • GenBank ID
    NM_024095.5
  • Entrez Gene
    ASB8 (a.k.a. PP14212)
  • Promoter EF1A
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-puro-EF1A-3XFLAG-hASB8_ANK3-4 was a gift from Dirk Daelemans (Addgene plasmid # 242468 ; http://n2t.net/addgene:242468 ; RRID:Addgene_242468)

  • For your References section:

    SINE compounds activate exportin 1 degradation through an allosteric mechanism. Wing CE, Fung HYJ, Kwanten B, Cagatay T, Niesman AB, Jacquemyn M, Gharghabi M, Permentier B, Shakya B, Nandi R, Ready JM, Kashyap T, Shacham S, Landesman Y, Lapalombella R, Daelemans D, Chook YM. Nat Chem Biol (2025) 21, 2002–2013. 10.1038/s41589-025-02058-0 PubMed 39416201
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