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pYO1879
(Plasmid #242480)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242480 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBluescript (KS-)
  • Vector type
    Bacterial Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPS21, LEU2
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Q66L
  • Tag / Fusion Protein
    • 2xDsRedExpress (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYO1879 was a gift from Roger Williams (Addgene plasmid # 242480 ; http://n2t.net/addgene:242480 ; RRID:Addgene_242480)

  • For your References section:

    A novel RAB5 binding site in human VPS34-CII that is likely the primordial site in eukaryotic evolution. Špokaitė S, Ohashi Y, Bourguet M, Dessus AN, Williams RL. eLife 10.7554/eLife.110040.1
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