pDASH-DIS-α2
(Plasmid
#242520)
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Purpose(Empty Backbone) Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, with attBTT and attBCC-FRT
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepDGB3alpha2
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Vector typeSynthetic Biology
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Selectable markersSacB
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://benchling.com/s/seq-9FZulVPxEh5HSkUPb5jt?m=slm-cajDx5fvvmXqPZCHUNsg for annotated plasmid map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDASH-DIS-α2 was a gift from Jose Alonso & Anna Stepanova (Addgene plasmid # 242520 ; http://n2t.net/addgene:242520 ; RRID:Addgene_242520) -
For your References section:
DASH: a versatile and high-capacity gene stacking system for plant synthetic biology. Zhao C, Stepanova AN, Alonso JM. Plant Biotechnol J. 2025 Jun 10. doi: 10.1111/pbi.70179. 10.1111/pbi.70179 PubMed 40493021