pPD0009_CROP-seq-mCherry2
(Plasmid
#242529)
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PurposeGeneral screening
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242529 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneLentiGuide-Puro
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Vector typeLentiviral
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Selectable markersPuromycin ; mCherry2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry2
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SpeciesOther
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPD0009_CROP-seq-mCherry2 was a gift from Christoph Bock & Paul Datlinger (Addgene plasmid # 242529 ; http://n2t.net/addgene:242529 ; RRID:Addgene_242529) -
For your References section:
Systematic discovery of CRISPR-boosted CAR T cell immunotherapies. Datlinger P, Pankevich EV, Arnold CD, Pranckevicius N, Lin J, Romanovskaia D, Schaefer M, Piras F, Orts AC, Nemc A, Biesaga PN, Chan M, Neuwirth T, Artemov AV, Li W, Ladstatter S, Krausgruber T, Bock C. Nature. 2025 Oct;646(8086):963-972. doi: 10.1038/s41586-025-09507-9. Epub 2025 Sep 24. 10.1038/s41586-025-09507-9 PubMed 40993398