p3E-IRES-Luciferase-WPRE-bGH-pA (JDW 1509)
(Plasmid
#242572)
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PurposeGateway 3' entry clone containing an IRES Luciferase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepDONR-p2r-P3
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Vector typeGateway subcloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIRES-Luciferase-WPRE-bGH-pA
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SpeciesSynthetic
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3E-IRES-Luciferase-WPRE-bGH-pA (JDW 1509) was a gift from Joshua Wythe (Addgene plasmid # 242572 ; http://n2t.net/addgene:242572 ; RRID:Addgene_242572) -
For your References section:
Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems. Gillespie W, Zhang Y, Ruiz OE, Cerda J, Ortiz-Guzman J, Turner WD, Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien CB, Kwan KM, Arenkiel BR, Devine WP, Wythe JD. bioRxiv [Preprint]. 2024 Jul 13:2024.07.13.603267. doi: 10.1101/2024.07.13.603267. 10.1101/2024.07.13.603267 PubMed 39026881