pEM790
(Plasmid
#242607)
-
Purpose(Empty Backbone) T-DNA plasmid, Level 2 destination vector, replaces pMIN-VS1
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242607 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepMIN-VS1
- Backbone size (bp) 6411
-
Vector typePlant Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Growth instructionsKanamycin 50mg/L and Chloramphenicol 25mg/L (grows slowly on Kan and Chlor). For maintenance, can use only Kanamycin 50mg/L
-
Copy numberHigh Copy
Cloning Information
- Cloning method Golden Gate
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid replaces the pMIN-VS1 Level 2 backbone in the linked publication (Addgene # 197783). pEM790 contains the 'overdrive' sequence that was accidentally omitted from pMIN-VS1. pMIN-VS1 is still functional, but we find fewer transformants using pMIN-VS1 when compared to pEM790. For cloning, pEM790 functions exactly the same way as pMIN-VS1.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEM790 was a gift from Daniel Voytas (Addgene plasmid # 242607 ; http://n2t.net/addgene:242607 ; RRID:Addgene_242607) -
For your References section:
An extensible vector toolkit and parts library for advanced engineering of plant genomes. Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R, Gamo ME, Starker CG, Zhang F, Voytas DF. Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312. 10.1002/tpg2.20312 PubMed 36896468
