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JARID1D-HE-AA
(Plasmid #242617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242617 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pFLAG-CMV2
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 9149
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JARID1D-HE-AA
  • Alt name
    KDM5D
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4449
  • GenBank ID
    NM_001146706.2
  • Entrez Gene
    KDM5D (a.k.a. HY, HYA, JARID1D, SMCY)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JARID1D-HE-AA was a gift from Min Gyu Lee (Addgene plasmid # 242617 ; http://n2t.net/addgene:242617 ; RRID:Addgene_242617)

  • For your References section:

    JARID1D Is a Suppressor and Prognostic Marker of Prostate Cancer Invasion and Metastasis. Li N, Dhar SS, Chen TY, Kan PY, Wei Y, Kim JH, Chan CH, Lin HK, Hung MC, Lee MG. Cancer Res. 2016 Feb 15;76(4):831-43. doi: 10.1158/0008-5472.CAN-15-0906. Epub 2016 Jan 8. 10.1158/0008-5472.CAN-15-0906 PubMed 26747897
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