pCMV-pLenti-ACTA2-R179 (WT) (LLH661)
(Plasmid
#242682)
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PurposePlasmid for expression of ACTA2-R179 (wild-type) cDNA from a lentiviral cassette
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCMV-pLenti
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepLenti-ACTA2-R179_cDNA
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Alt nameLLH661
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Alt namepLenti-ACTA2-R179 (WT)
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-pLenti-ACTA2-R179 (WT) (LLH661) was a gift from Benjamin Kleinstiver (Addgene plasmid # 242682 ; http://n2t.net/addgene:242682 ; RRID:Addgene_242682) -
For your References section:
Treatment of a severe vascular disease using a bespoke CRISPR-Cas9 base editor in mice. Alves CRR, Das S, Krishnan V, Ha LL, Fox LR, Stutzman HE, Shamber CE, Kalailingam P, McCarthy S, Lino Cardenas CL, Fong CE, Imai T, Mitra S, Yun S, Wood RK, Benning FMC, Roh K, Lawton J, Kim N, Silverstein RA, Ferreira da Silva J, de la Cruz D, Richa R, Xie J, Gray-Edwards HL, Malhotra R, Chung DY, Chao LH, Tsai SQ, Maguire CA, Lindsay ME, Kleinstiver BP, Musolino PL. Nat Biomed Eng. 2025 Sep 11:10.1038/s41551-025-01499-1. doi: 10.1038/s41551-025-01499-1. 10.1038/s41551-025-01499-1 PubMed 40935887