pCMV-PE2-SpG (HES824)
(Plasmid
#242689)
-
PurposePrime editing in mammalian cells with SpG-based PE2 prime editors
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 242689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepCMV-PE2 (Addgene Plasmid #132775)
-
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepCMV-NLS-SpCas9(H840A)-M-MLV-RT(D200N,T306K,W313F,T330P,L603W)-NLS
-
Alt nameHES824
-
Alt namepCMV-PE2-SpG
-
SpeciesSynthetic
-
MutationPE2 mutations in MMLV RT, SpG mutations in SpCas9(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R)
- Promoter CMV
-
Tag
/ Fusion Protein
- NLS (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer oBK6928-CCAAGTCTCCACCCCATTGACG
- 3′ sequencing primer oBK219-GGGAGTGGCACCTTCCAGGGTC (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-PE2-SpG (HES824) was a gift from Benjamin Kleinstiver (Addgene plasmid # 242689 ; http://n2t.net/addgene:242689 ; RRID:Addgene_242689)