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pLenti-HA-CHAC1
(Plasmid #242710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242710 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro
  • Backbone manufacturer
    Kazuhiro Oka, Addgene plasmid #73037
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHAC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    790
  • Mutation
    Multiple wobble mutations
  • Entrez Gene
    CHAC1
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer common
  • 3′ sequencing primer common
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Containing T2A self-cleaving site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-HA-CHAC1 was a gift from Xingguo Zhu (Addgene plasmid # 242710 ; http://n2t.net/addgene:242710 ; RRID:Addgene_242710)
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