pXeDNA3.1-LacZ
(Plasmid
#242746)
-
Purpose(Empty Backbone) pXeDNA3-LacZ TA-cloning vector for dual expression: CMV enhancer/promoter in mammalian cells and Xenopus β-globin 5'/3'UTRs in oocytes; BGHpA, AmpR, NeoR, lacZα for blue-white screening.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepXeDNA3.1(pcDNA3.1-based)
-
Backbone manufacturerLab-constructed
- Backbone size (bp) 5966
-
Modifications to backboneInserted Xenopus β-globin 5' & 3' UTRs, replaced MCS with lacZα-TA site for blue-white TA-cloning, added upstream T7 promoter; CMV promoter/BGH polyA, AmpR & NeoR retained.
-
Vector typeMammalian Expression ; Xenopus oocyte expression
- Promoter CMV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pXeDNA3.1-LacZ was a gift from Luis Yanez-Guerra (Addgene plasmid # 242746 ; http://n2t.net/addgene:242746 ; RRID:Addgene_242746)
