pAAV-CAG-eGFP-Tag100
(Plasmid #242779)

• Purpose: AAV transfer plasmid expressing eGFP-Tag100 under a CAG promoter.
• Depositing Lab: Andrew Payne
• Publication: Park et al bioRxiv 2025.09.26.678648

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Backbone

• Vector backbone: pAAV-CAG-GFP (Addgene Plasmid 37825)
• Backbone manufacturer: Edward Boyden
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Species: Synthetic
• Promoter: CAG
• Tag / Fusion Protein:
  • Tag100 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pAAV-CAG-eGFP-Tag100.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.26.678648 for bioRxiv preprint.

There is a minor discrepancy between the Addgene NGS sequence and the depositor sequence: 1bp indel in R-ITR. Depositor confirms this is not of concern for plasmid function.