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FUGW-EGFP-mPkm2-T328A
(Plasmid #242813)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242813 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    FUGW
  • Backbone size w/o insert (bp) 9221
  • Total vector size (bp) 11534
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pyruvate kinase, muscle
  • Alt name
    Pkm2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1596
  • Mutation
    T328A
  • Entrez Gene
    Pkm (a.k.a. Pk-2, Pk-3, Pk3, Pkm2)
  • Promoter Ubiquitin C
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUGW-EGFP-mPkm2-T328A was a gift from Ronald Hart (Addgene plasmid # 242813 ; http://n2t.net/addgene:242813 ; RRID:Addgene_242813)

  • For your References section:

    Alpha-ketoglutarate mitigates insulin resistance and metabolic inflexibility in a mouse model of Ataxia-Telangiectasia. Sun JK, Hart RP, Herrup K, Peng AZ, Wong GC, Wu D, Kwan KM, Chow KH. Nat Commun. 2025 Oct 21;16(1):9312. doi: 10.1038/s41467-025-64360-8. 10.1038/s41467-025-64360-8 PubMed 41120320
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