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His6-tev-G(TTR F87E)
(Plasmid #242846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242846 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET21d
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5440
  • Total vector size (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    462
  • Mutation
    M0G, N-terminal methionine mutated to glycine, F87E
  • Entrez Gene
    TTR (a.k.a. ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN)
  • Promoter T7
  • Tag / Fusion Protein
    • His6tev-G (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jeffery Kelly lab; we made mutations on the wild type construct received from the Kelly lab. Description of wild type TTR plasmid in Zhang et al. 2014 ACS Chem Biol (https://doi.org/10.1021/cb5004477).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6-tev-G(TTR F87E) was a gift from Peter Wright (Addgene plasmid # 242846 ; http://n2t.net/addgene:242846 ; RRID:Addgene_242846)

  • For your References section:

    Initiation of transthyretin aggregation at neutral pH by fluid agitation. Ritsch I, Dyson HJ, Wright PE. Proc Natl Acad Sci U S A. 2025 Mar 18;122(11):e2425230122. doi: 10.1073/pnas.2425230122. Epub 2025 Mar 11. 10.1073/pnas.2425230122 PubMed 40067885
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