pFS557
(Plasmid
#242857)
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PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCR2.1-hph
- Backbone size w/o insert (bp) 5632
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Vector typeYeast Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameTetR
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)621
- Promoter CMV promoter
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemECitrine
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)714
- Promoter enotetSW1 promoter
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFS557 was a gift from Nick Rhind (Addgene plasmid # 242857 ; http://n2t.net/addgene:242857 ; RRID:Addgene_242857)