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Addgene

pU6 pegRNA AAV 4a PhiC31 attP41
(Plasmid #242882)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242882 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pU6 pegRNA GG Acceptor
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    epegRNA for PhiC31 attP site
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Promoter U6

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

please email [email protected] each time it is ordered.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6 pegRNA AAV 4a PhiC31 attP41 was a gift from Jesse Owens (Addgene plasmid # 242882 ; http://n2t.net/addgene:242882 ; RRID:Addgene_242882)

  • For your References section:

    Directed evolution of hyperactive integrases for site specific insertion of transgenes. Hew BE, Gupta S, Sato R, Waller DF, Stoytchev I, Short JE, Sharek L, Tran CT, Badran AH, Owens JB. Nucleic Acids Res. 2024 Jul 2:gkae534. doi: 10.1093/nar/gkae534. 10.1093/nar/gkae534 PubMed 38953167
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