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Addgene

pCMV Bxb1 attP dsRed Hygro
(Plasmid #242884)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242884 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hygromycin-IRE2S-DsRED Express 2
  • Species
    Synthetic
  • Mutation
    none
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

please email [email protected] each time it is ordered.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV Bxb1 attP dsRed Hygro was a gift from Jesse Owens (Addgene plasmid # 242884 ; http://n2t.net/addgene:242884 ; RRID:Addgene_242884)

  • For your References section:

    Directed evolution of hyperactive integrases for site specific insertion of transgenes. Hew BE, Gupta S, Sato R, Waller DF, Stoytchev I, Short JE, Sharek L, Tran CT, Badran AH, Owens JB. Nucleic Acids Res. 2024 Jul 2:gkae534. doi: 10.1093/nar/gkae534. 10.1093/nar/gkae534 PubMed 38953167
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