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LZRS-MS-NEO_PIWIL2-FLAG
(Plasmid #242890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242890 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    LZRS-MS-NEO
  • Backbone size w/o insert (bp) 12972
  • Total vector size (bp) 15991
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PIWIL2
  • Alt name
    HILI
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2919
  • GenBank ID
    NM_001135721
  • Entrez Gene
    PIWIL2 (a.k.a. CT80, HILI, PIWIL1L, mili)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LZRS-MS-NEO_PIWIL2-FLAG was a gift from Antonis Kourtidis (Addgene plasmid # 242890 ; http://n2t.net/addgene:242890 ; RRID:Addgene_242890)

  • For your References section:

    PIWIL2 downregulation in colon cancer promotes transposon activity and pro-tumorigenic phenotypes. Risner A, Nair-Menon J, Cheedipudi A, Delaney JR, Gangaraju V, Kourtidis A. Biol Open. 2025 Aug 11:bio.061942. doi: 10.1242/bio.061942. 10.1242/bio.061942 PubMed 40789164
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