Skip to main content
Addgene
Open Menu

pV1210
(Plasmid #242891)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242891 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pV1200
  • Backbone manufacturer
    Valmik Vyas
  • Total vector size (bp) 13113
  • Modifications to backbone
    Replaced C. albicans ENO1promoter, ENO1terminator and SNR52promoter with C. auris sequences
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    100µg/mL Ampicillin in LB medium or 50µg/mL Nourseothricin in LB medium for bacterial propagation.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CaCas9/sgRNA-BsmBI stuffer
  • gRNA/shRNA sequence
    GGAGACGGAATTCCGTCTC - sgRNA-BsmBI stuffer
  • Species
    Synthetic; Candida auris, Candida albicans, Streptococcus pyogenes

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.09.632232 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pV1210 was a gift from Patrick Van Dijck (Addgene plasmid # 242891 ; http://n2t.net/addgene:242891 ; RRID:Addgene_242891)

  • For your References section:

    A comparative evaluation of CRISPR-Cas9 allele editing systems in Candida auris: challenging research in a challenging bug. Sofras D, Carolus H, Subotic A, Romero CL, Ennis CL, Hernday AD, Nobile CJ, Rybak JM, Van Dijck P. bioRxiv [Preprint]. 2025 Jan 11:2025.01.09.632232. doi: 10.1101/2025.01.09.632232. 10.1101/2025.01.09.632232 PubMed 39829791
Related items: