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PB_rtTA_BsmB-HNRNPK_sgRNA_1
(Plasmid #242897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242897 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PB_rtTA_BsmBI
  • Backbone manufacturer
    Mauro Calabrese (Addgene plasmid # 126028)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HNRNPK
  • gRNA/shRNA sequence
    AAACTTACCCCCCTCTGG
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Hnrnpk (a.k.a. Hnrpk, KBBP, NOVA)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Knockdown validated by Western blot in Trophpblast Stem Cells

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB_rtTA_BsmB-HNRNPK_sgRNA_1 was a gift from Mauro Calabrese (Addgene plasmid # 242897 ; http://n2t.net/addgene:242897 ; RRID:Addgene_242897)

  • For your References section:

    lncRNA-Induced Spread of Polycomb Controlled by Genome Architecture, RNA Abundance, and CpG Island DNA. Schertzer MD, Braceros KCA, Starmer J, Cherney RE, Lee DM, Salazar G, Justice M, Bischoff SR, Cowley DO, Ariel P, Zylka MJ, Dowen JM, Magnuson T, Calabrese JM. Mol Cell. 2019 Aug 8;75(3):523-537.e10. doi: 10.1016/j.molcel.2019.05.028. Epub 2019 Jun 27. 10.1016/j.molcel.2019.05.028 PubMed 31256989
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