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PB_rtTA_BsmB-HNRNPU_sgRNA_3
(Plasmid #242902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242902 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PB_rtTA_BsmBI
  • Backbone manufacturer
    Mauro Calabrese (Addgene plasmid # 126028)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HNRNPU
  • gRNA/shRNA sequence
    AACGGCAGTCTCGACCTAGG
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Hnrnpu (a.k.a. Hnrpu, SAFA, Sp120, hnRNP U)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Knockdown validated by Western blot in Trophpblast stem cells. Please visit https://doi.org/10.1101/2025.07.16.665154 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB_rtTA_BsmB-HNRNPU_sgRNA_3 was a gift from Mauro Calabrese (Addgene plasmid # 242902 ; http://n2t.net/addgene:242902 ; RRID:Addgene_242902)

  • For your References section:

    Correlated protein-RNA associations reveal a requirement for HNRNPU in long-range Polycomb recruitment by the lncRNAs Airn, Kcnq1ot1, and Xist. Murvin MM, Li S, Boyson SP, Zhang Z, Cherney RE, Calabrese JM. bioRxiv 2025.07.16.665154 10.1101/2025.07.16.665154
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