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Addgene

pCK660
(Plasmid #242914)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242914 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCD442
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PspF-λN22
  • Species
    Synthetic
  • Entrez Gene
    pspF (a.k.a. b1303, ECK1298, ycjB)
  • Promoter BBa_J23107

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CACGCATTGATTTGAGTCAGC
  • 3′ sequencing primer tcgtaagccatttccgctcg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.14.654134 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCK660 was a gift from Jesse Zalatan (Addgene plasmid # 242914 ; http://n2t.net/addgene:242914 ; RRID:Addgene_242914)

  • For your References section:

    Systematic Mapping of Bacterial CRISPRa Systems for Synergistic Gene Activation Reveals Antagonistic Effects. Kiattisewee C, Karanjia AV, Cardiff RAL, Olander KE, Leejareon P, Alvi SS, Carothers JM, Zalatan JG. ACS Synth Biol. 2025 Jul 22. doi: 10.1021/acssynbio.5c00358. 10.1021/acssynbio.5c00358 PubMed 40693287
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