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pDL1-Spol-Ovo-Nterm
(Plasmid #242942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242942 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDL1
  • Backbone manufacturer
    Daniel Lobo (Addgene plasmid # 182263)
  • Backbone size w/o insert (bp) 2319
  • Total vector size (bp) 2593
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spol-Ovo (Nterm fragment)
  • Species
    Schmidtea polychroa
  • Insert Size (bp)
    400

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCAGCACTCTACTCGCAAAC
  • 3′ sequencing primer CGGAGGTTTTCAGACACGGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDL1-Spol-Ovo-Nterm was a gift from Erin Davies (Addgene plasmid # 242942 ; http://n2t.net/addgene:242942 ; RRID:Addgene_242942)

  • For your References section:

    Protocol combining RNA interference and regeneration assays in planarian embryos. Deihl EW, Booth CLT, Davies EL. bioRxiv [Preprint]. 2025 Jul 22:2025.07.21.666018. doi: 10.1101/2025.07.21.666018. 10.1101/2025.07.21.666018 PubMed 40777484
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