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Addgene

GFP-Nsp3(1-111)
(Plasmid #242947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242947 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET
  • Backbone size w/o insert (bp) 5251
  • Total vector size (bp) 6383
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-Nsp3(1-111)
  • Alt name
    Non Structural Protein 3
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    1134
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Nsp3(1-111) was a gift from Benjamin Schuster (Addgene plasmid # 242947 ; http://n2t.net/addgene:242947 ; RRID:Addgene_242947)

  • For your References section:

    Phosphorylation Toggles the SARS-CoV-2 Nucleocapsid Protein Between Two Membrane-Associated Condensate States. Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Gormley AJ, Murthy S, Dignon G, Soranno A, Shi Z, Schuster BS. bioRxiv [Preprint]. 2025 Jun 10:2024.10.17.618867. doi: 10.1101/2024.10.17.618867. 10.1101/2024.10.17.618867 PubMed 39464032
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