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pRS316 Gal1p1 IpgA
(Plasmid #242952)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242952 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRS316
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Ampiillin concentration is 100 μg/mL
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IpgA (IcsB Chaperone)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    387
  • Tag / Fusion Protein
    • Myc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GAATTC
  • 3′ sequencing primer GGATCC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS316 Gal1p1 IpgA was a gift from François-Xavier Campbell-Valois (Addgene plasmid # 242952 ; http://n2t.net/addgene:242952 ; RRID:Addgene_242952)

  • For your References section:

    A bacterial family of fatty acid acyltransferases related to the Shigella effector IcsB. Bajunaid W, Tomaro K, Kottarampatel AH, Desrochers GF, Lavallée-Adam M, Pezacki JP, Campbell-Valois F.. mBio 0:e03890-25 10.1128/mbio.03890-25
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