pAG AAV CMV EGFP
(Plasmid
#242959)
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PurposeAAV plasmid expressing EGFP, control for plasmid 242957
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242959 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * | |
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Backbone
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Vector backbonepAG AAV CMV Empty
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Backbone manufacturerAddgene Research Program (Addgene plasmid # 242957)
- Backbone size w/o insert (bp) 3849
- Total vector size (bp) 4566
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesSynthetic
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Insert Size (bp)717
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BstBI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer gtaacaactccgccccatt (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Control plasmid for empty backbone 242957 pAG AAV CMV Empty.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG AAV CMV EGFP was a gift from Meghan Rego & Addgene Research Program (Addgene plasmid # 242959 ; http://n2t.net/addgene:242959 ; RRID:Addgene_242959)