pAG AAV CMV EGFP-HA
(Plasmid
#242963)
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PurposeEdit AAV plasmid expressing EGFP-HA, control for plasmid 242962
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242963 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * | |
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Backbone
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Vector backbonepAG AAV
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 3879
- Total vector size (bp) 4596
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesSynthetic
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Insert Size (bp)709
- Promoter CMV
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BstBI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer gtaacaactccgccccatt (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Control for plasmid 242962 pAG AAV CMV C-HA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG AAV CMV EGFP-HA was a gift from Meghan Rego & Addgene Research Program (Addgene plasmid # 242963 ; http://n2t.net/addgene:242963 ; RRID:Addgene_242963)