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pEF1a_ABE9-SpRY
(Plasmid #242977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242977 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEF1a, custom (Addgene plasmid #174824, pEF1a-hMLH1dn)
  • Backbone size w/o insert (bp) 4641
  • Total vector size (bp) 8859
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pEF1α-TadA-8e (N108Q/L145T)-nSpRYCas9
  • Alt name
    pEF1a_ABE9-SpRY
  • Species
    Synthetic
  • Insert Size (bp)
    4818
  • Promoter EF1a
  • Tag / Fusion Protein
    • 6*His (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.07.680931 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a_ABE9-SpRY was a gift from Marc Freichel (Addgene plasmid # 242977 ; http://n2t.net/addgene:242977 ; RRID:Addgene_242977)

  • For your References section:

    ABE9 fused to SpRY Cas9 nickase enables precise generation of bystander free mouse models. Ong JK, Bhunia S, Hilbert B, Kirschner V, Duglosz S, Zimmermann F, Freichel M, Cornean A. Sci Rep (2026). https://doi.org/10.1038/s41598-026-40642-z 10.1038/s41598-026-40642-z
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