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pPD0035_CROPseq-CAR-Puro_CD19-28z
(Plasmid #242983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242983 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    LentiGuide-Puro
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD19-28z FMC63 CAR
  • Species
    H. sapiens (human)
  • Entrez Gene
    CD19 (a.k.a. B4, CVID3)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPD0035_CROPseq-CAR-Puro_CD19-28z was a gift from Christoph Bock & Paul Datlinger (Addgene plasmid # 242983 ; http://n2t.net/addgene:242983 ; RRID:Addgene_242983)

  • For your References section:

    Systematic discovery of CRISPR-boosted CAR T cell immunotherapies. Datlinger P, Pankevich EV, Arnold CD, Pranckevicius N, Lin J, Romanovskaia D, Schaefer M, Piras F, Orts AC, Nemc A, Biesaga PN, Chan M, Neuwirth T, Artemov AV, Li W, Ladstatter S, Krausgruber T, Bock C. Nature. 2025 Oct;646(8086):963-972. doi: 10.1038/s41586-025-09507-9. Epub 2025 Sep 24. 10.1038/s41586-025-09507-9 PubMed 40993398
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