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pSBtet-mCer3-TGN38-FAP
(Plasmid #242994)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242994 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSBtet-puro
  • Backbone size w/o insert (bp) 5581
  • Total vector size (bp) 7462
  • Vector type
    Mammalian Expression ; Sleeping Beauty
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCer3-TGN38-dH6.2
  • Alt name
    mCer3-TGN38-FAP
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1881
  • Entrez Gene
    Tgoln2 (a.k.a. Tgn38, Tgoln1, Ttgn1)
  • Promoter Tight TRE
  • Tags / Fusion Proteins
    • mCer3 (N terminal on insert)
    • FAP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCCTGGAGCCAATTCCAACTC
  • 3′ sequencing primer GTCTATCGATGGAAGCTTGGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBtet-mCer3-TGN38-FAP was a gift from Itay Budin (Addgene plasmid # 242994 ; http://n2t.net/addgene:242994 ; RRID:Addgene_242994)

  • For your References section:

    Leaflet-specific phospholipid imaging using genetically encoded proximity sensors. Moore WM, Brea RJ, Knittel C, Wrightsman E, Hui B, Lou J, Ancajas CF, Best MD, Obara, CJ Devaraj NK, Budin I. Nat Chem Biol (2025). https://doi.org/10.1038/s41589-025-02021-z. 10.1038/s41589-025-02021-z PubMed 38746395
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