|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24306||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerNaldini/Trono (Addgene #12252)
- Backbone size w/o insert (bp) 6132
Growth in Bacteria
Copy numberHigh Copy
Alt namefirefly luciferase
Insert Size (bp)1919
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer GGCCCGAAGGAATAGAAG
- 3′ sequencing primer AACGGGCCACAACTCCTC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe 7xTcf promoter is derived from pSuperTOPflash, published in Veeman MT et al., Curr Biol 13: 680–685 (2003)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
TFC repeat sequence is AGATCAAAGGgg
This plasmid requires a 2nd generation lentiviral packaging system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:7TF was a gift from Roel Nusse (Addgene plasmid # 24306 ; http://n2t.net/addgene:24306 ; RRID:Addgene_24306)
For your References section:Lentiviral vectors to probe and manipulate the wnt signaling pathway. Fuerer C, Nusse R. PLoS One. 2010 . 5(2):e9370. 10.1371/journal.pone.0009370 PubMed 20186325