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ptubP-QS
(Plasmid #24352)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24352 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCasper-tubulin-GAL80
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    QS
  • Species
    Neurospora crassa
  • Insert Size (bp)
    2500
  • Tag / Fusion Protein
    • white gene (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Acc65I (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer pCasper-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The QS cDNA was excised from pAC-QS with Acc65I and NotI, blunted, and ligated into
a blunted pCasper-tubulin-GAL80, from which the GAL80 insert was removed by digestion with
NotI/XbaI.

There is a ~150bp deletion in the SV40 tail compared to the pUAST-SV40 sequence from Flybase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptubP-QS was a gift from Liqun Luo (Addgene plasmid # 24352 ; http://n2t.net/addgene:24352 ; RRID:Addgene_24352)
  • For your References section:

    The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990