|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24352||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10000
Vector typeInsect Expression
Growth in Bacteria
Insert Size (bp)2500
/ Fusion Protein
- white gene (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Acc65I (destroyed during cloning)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer pCasper-F (Common Sequencing Primers)
The QS cDNA was excised from pAC-QS with Acc65I and NotI, blunted, and ligated into
a blunted pCasper-tubulin-GAL80, from which the GAL80 insert was removed by digestion with
There is a ~150bp deletion in the SV40 tail compared to the pUAST-SV40 sequence from Flybase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ptubP-QS was a gift from Liqun Luo (Addgene plasmid # 24352 ; http://n2t.net/addgene:24352 ; RRID:Addgene_24352)
For your References section:The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990