|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24364||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8000
Vector typeInsect Expression
Growth in Bacteria
Insert Size (bp)2500
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site AatII (not destroyed)
- 5′ sequencing primer pCasper-F (Common Sequencing Primers)
An enhancer trap was constructed that allows
recombination mediated cassette exchange (Oberstein et al., 2005). A loxM2 site was inserted
immediately following the 5P promoter, and a LoxP site was placed after the selectable white
marker to generate the vector pDonor-M2, which contains elements in the following order: 5P-
Ppromoter-LoxM2-MCS-SV40 terminator-white-LoxP-3P. QF was PCR amplified from pAC-QF
adding 5’ EcoRI and 3’ AatII restriction sites, and insertedinto the EcoRI/AatII cloning sites of pDonor-M2 to yield pQF-M2ET.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQF-M2ET was a gift from Liqun Luo (Addgene plasmid # 24364 ; http://n2t.net/addgene:24364 ; RRID:Addgene_24364)
For your References section:The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990