TB6PDGH-pNIC-Bsa4
(Plasmid
#243657)
-
PurposeExpresses trypansoma brucei 6 phosphogluconate dehydrogenase under T7 with HIS tag in bacterial cells
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 243657 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepNIC-Bsa4
-
Backbone manufacturerSGC
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 6400
-
Vector typeBacterial Expression
-
Selectable markers6xHIS tag
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameTrypansoma brucei 6-phophogluconate dehydrogenase
-
Alt nameTB6PDGH
-
SpeciesT.brucei
-
Insert Size (bp)1200
-
Tag
/ Fusion Protein
- 6xHIS (N terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
TB6PDGH-pNIC-Bsa4 was a gift from Josh Beckham (Addgene plasmid # 243657 ; http://n2t.net/addgene:243657 ; RRID:Addgene_243657)