pGRF-GIF
(Plasmid
#243677)
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PurposeExpresses a chimeric protein composed of the wheat GROWTH-REGULATING-FACTOR and its wheat cofactor GRF-INTERACTING-FACTOR1 in plants via the CmYLCV 9.11 promoter and an Arabidopsis HSP terminator
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 243677 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC-GW-Kan
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Backbone manufacturerGenewiz
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChimeric protein composed of GROWTH-REGULATING-FACTOR and its cofactor GRF-INTERACTING-FACTOR1 from wheat
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Alt nameGRF-GIF
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SpeciesWheat
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGRF-GIF was a gift from Patrick Krysan (Addgene plasmid # 243677 ; http://n2t.net/addgene:243677 ; RRID:Addgene_243677) -
For your References section:
Efficient production of gene-edited onion (Allium cepa) plants using biolistic delivery of cas9 RNPs and transient expression constructs. De La Mora CL, Havey MJ, Krysan PJ. Plant Cell Rep. 2025 Oct 19;44(11):243. doi: 10.1007/s00299-025-03626-3. 10.1007/s00299-025-03626-3 PubMed 41110011