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pJL309
(Plasmid #243705)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243705 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pJL239
  • Backbone manufacturer
    Weissman Lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NDUFB9
  • Species
    H. sapiens (human)
  • Mutation
    mitochondrial targeting sequence deleted
  • Entrez Gene
    NDUFB9 (a.k.a. B22, CI-B22, LYRM3, UQOR22)
  • Tag / Fusion Protein
    • mNeonGreen2(11) (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL309 was a gift from Jonathan Weissman (Addgene plasmid # 243705 ; http://n2t.net/addgene:243705 ; RRID:Addgene_243705)

  • For your References section:

    Proximity-specific ribosome profiling reveals the logic of localized mitochondrial translation. Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, Ting AY, Weissman JS. Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002. 10.1016/j.cell.2025.08.002 PubMed 40876456
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