Skip to main content
Addgene
Open Menu

pJL388
(Plasmid #243717)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243717 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pJL349
  • Backbone manufacturer
    Weissman Lab
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AKAP1
  • Species
    H. sapiens (human)
  • Mutation
    GXXG motif (GKQG 624-627) mutated to GDDG, and gRNA-resistant silent mutations
  • Entrez Gene
    AKAP1 (a.k.a. AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, SAKAP84, TDRD17)
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL388 was a gift from Jonathan Weissman (Addgene plasmid # 243717 ; http://n2t.net/addgene:243717 ; RRID:Addgene_243717)

  • For your References section:

    Proximity-specific ribosome profiling reveals the logic of localized mitochondrial translation. Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, Ting AY, Weissman JS. Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002. 10.1016/j.cell.2025.08.002 PubMed 40876456
Related items: