pJH106
(Plasmid
#243727)
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PurposeExpresses a chimeric form of PMPCA, where the region between residue 100 and 351 is replaced by a 250-residue XTEN linker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 243727 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepJL163
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Backbone manufacturerWeissman Lab
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePMPCA(1-100aa)-XTEN250-PMPCA(351aa-stop)
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SpeciesH. sapiens (human)
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MutationThe region between residue 100 and 351 of PMPCA is replaced by a 250-residue XTEN linker
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Entrez GenePMPCA (a.k.a. Alpha-MPP, CLA1, CPD3, INPP5E, MAS2, P-55, SCAR2)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH106 was a gift from Jonathan Weissman (Addgene plasmid # 243727 ; http://n2t.net/addgene:243727 ; RRID:Addgene_243727) -
For your References section:
Proximity-specific ribosome profiling reveals the logic of localized mitochondrial translation. Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, Ting AY, Weissman JS. Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002. 10.1016/j.cell.2025.08.002 PubMed 40876456