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pJH122
(Plasmid #243728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243728 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pJL163
  • Backbone manufacturer
    Weissman Lab
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALDH18A1(1-97aa)-XTEN700
  • Species
    H. sapiens (human)
  • Mutation
    The remainder of ALDH18A1 after the first 97 residues is replaced by a 700-residue XTEN linker
  • Entrez Gene
    ALDH18A1 (a.k.a. ADCL3, ARCL3A, GSAS, P5CS, PYCS, SPG9, SPG9A, SPG9B)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH122 was a gift from Jonathan Weissman (Addgene plasmid # 243728 ; http://n2t.net/addgene:243728 ; RRID:Addgene_243728)

  • For your References section:

    Proximity-specific ribosome profiling reveals the logic of localized mitochondrial translation. Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, Ting AY, Weissman JS. Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002. 10.1016/j.cell.2025.08.002 PubMed 40876456
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