pShuttle-EGFP-CLASP2 340-1362 9xS/A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24373||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerAgilent Technologies
- Backbone size w/o insert (bp) 7469
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Gene/Insert nameCLASP2 (340-1362) 9xS/A
SpeciesH. sapiens (human)
Insert Size (bp)3800
MutationNonphosphorylatable CLASP2 deletion mutant. Mutation of serines in GSK3β motif between S594 to S614 plus S568/572/576A.
/ Fusion Protein
- EGFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV-F, EGFP-C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pShuttle-EGFP-CLASP2 340-1362 9xS/A was a gift from Torsten Wittmann (Addgene plasmid # 24373 ; http://n2t.net/addgene:24373 ; RRID:Addgene_24373)
For your References section:GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T. J Cell Biol. 2009 Mar 23. 184(6):895-908. 10.1083/jcb.200901042 PubMed 19289791