pShuttle-mRFP-gsk3 s9a
(Plasmid #24371)

• Depositing Lab: Torsten Wittmann
• Publication: Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908.

Backbone

• Vector backbone: pShuttle-CMV
• Backbone manufacturer: Agilent Technologies
• Backbone size w/o insert (bp): 7469
• Vector type: Mammalian Expression, Adenoviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GSK3beta(S9A)
• Alt name: GSK3beta
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: S9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
• Entrez Gene: GSK3B
• Tag / Fusion Protein:
  • mRFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F, EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA obtained from Niels Galjart
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry