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pShuttle-mRFP-gsk3 s9a
(Plasmid #24371)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24371 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pShuttle-CMV
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 7469
  • Vector type
    Mammalian Expression, Adenoviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GSK3beta(S9A)
  • Alt name
    GSK3beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    S9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
  • Entrez Gene
    GSK3B
  • Tag / Fusion Protein
    • mRFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F, EGFP-C
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pShuttle-mRFP-gsk3 s9a was a gift from Torsten Wittmann (Addgene plasmid # 24371)
  • For your References section:

    GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T. J Cell Biol. 2009 Mar 23. 184(6):895-908. 10.1083/jcb.200901042 PubMed 19289791