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pAWP78-PVCpnf_pvc13-Ad5_pvc8-Cas9
(Plasmid #243737)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243737 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAWP78
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pvc13-Ad5_pvc8-Cas9
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains an A468T mutation in pvc15 ATPase, an E291G mutation in trfA, and a single nucleotide mutation in traJ. These mutations are not known to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAWP78-PVCpnf_pvc13-Ad5_pvc8-Cas9 was a gift from Feng Zhang (Addgene plasmid # 243737 ; http://n2t.net/addgene:243737 ; RRID:Addgene_243737)

  • For your References section:

    Targeted delivery of diverse biomolecules with engineered bacterial nanosyringes. Kreitz J, Yang V, Friedrich MJ, Pham J, Macrae RK, Zhang F. Nat Biotechnol. 2025 Aug 12. doi: 10.1038/s41587-025-02774-x. 10.1038/s41587-025-02774-x PubMed 40796978
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