TwinStrepSUMO-scFv-SpyCatcher
(Plasmid
#243753)
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PurposeFor affinity purification of anti-HA scFv fused to SpyCatcher
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 243753 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneTwinStrep-SUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namescFv-SpyCatcher
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TwinStrepSUMO-scFv-SpyCatcher was a gift from Feng Zhang (Addgene plasmid # 243753 ; http://n2t.net/addgene:243753 ; RRID:Addgene_243753) -
For your References section:
Targeted delivery of diverse biomolecules with engineered bacterial nanosyringes. Kreitz J, Yang V, Friedrich MJ, Pham J, Macrae RK, Zhang F. Nat Biotechnol. 2025 Aug 12. doi: 10.1038/s41587-025-02774-x. 10.1038/s41587-025-02774-x PubMed 40796978