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AAV-CAG-HA-mTFEB
(Plasmid #243758)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243758 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    CIGW
  • Backbone manufacturer
    Pamela McLean
  • Backbone size w/o insert (bp) 6443
  • Total vector size (bp) 7878
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Transcription factor EB, codon optimized
  • Alt name
    TFEB
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1461
  • Entrez Gene
    Tfeb (a.k.a. Tcfeb, bHLHe35)
  • Promoter CAG
  • Tag / Fusion Protein
    • HA tag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GCAAAGAATTCTCGAGCCACAATGTACCCTTACGACG
  • 3′ sequencing primer GGATCCGGTACCGATTCACTACAACACGTCTCCTTCTTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-CAG-HA-mTFEB was a gift from Andrew Arrant (Addgene plasmid # 243758 ; http://n2t.net/addgene:243758 ; RRID:Addgene_243758)

  • For your References section:

    TFEB overexpression alleviates autophagy-lysosomal deficits caused by progranulin insufficiency. Nader WO, Brown KS, Boyle NR, Kaplelach AK, Abdelaziz SM, Davis SE, Aljabi Q, Hakim AR, Davidson AG, Vollmer GA, Wright LC, Echols JB, Saad J, Pena NS, Arrant AE. Sci Rep. 2025 Jul 19;15(1):26217. doi: 10.1038/s41598-025-12268-0. 10.1038/s41598-025-12268-0 PubMed 40683940
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