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AAV-CAG-GRN-HA
(Plasmid #243760)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243760 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    CIGW
  • Backbone manufacturer
    Pamela McLean
  • Backbone size w/o insert (bp) 6443
  • Total vector size (bp) 8267
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Progranulin
  • Alt name
    GRN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1824
  • GenBank ID
    NM_002087
  • Entrez Gene
    GRN (a.k.a. CLN11, FTD2, GEP, GP88, PCDGF, PEPI, PGRN)
  • Promoter CAG
  • Tag / Fusion Protein
    • HA Tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GAATTCTGCAGTCGAGCCACCATGTGGACCCTG
  • 3′ sequencing primer GGATCCGGTACCGATTTAAGCGTAATCTGGAACATCGT
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Origene construct SKU RC202139

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-CAG-GRN-HA was a gift from Andrew Arrant (Addgene plasmid # 243760 ; http://n2t.net/addgene:243760 ; RRID:Addgene_243760)

  • For your References section:

    TFEB overexpression alleviates autophagy-lysosomal deficits caused by progranulin insufficiency. Nader WO, Brown KS, Boyle NR, Kaplelach AK, Abdelaziz SM, Davis SE, Aljabi Q, Hakim AR, Davidson AG, Vollmer GA, Wright LC, Echols JB, Saad J, Pena NS, Arrant AE. Sci Rep. 2025 Jul 19;15(1):26217. doi: 10.1038/s41598-025-12268-0. 10.1038/s41598-025-12268-0 PubMed 40683940
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