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pET24a-VHH-iRFP670
(Plasmid #243768)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243768 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET24a
  • Backbone manufacturer
    Merck - Novagen
  • Total vector size (bp) 6501
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    anti-GFP nanobody VHH
  • Alt name
    VHHGFP4
  • Species
    Synthetic
  • Insert Size (bp)
    1467
  • Promoter T7
  • Tags / Fusion Proteins
    • T7-TEV site (N terminal on insert)
    • iRFP670-HA-AviTag-6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET24a-VHH-iRFP670 was a gift from Dominik Buser & Martin Spiess (Addgene plasmid # 243768 ; http://n2t.net/addgene:243768 ; RRID:Addgene_243768)

  • For your References section:

    Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells. Buser DP, Schleicher KD, Junne T, Biehlmaier O. Jove Journal 2025 10.3791/69284
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