mGreenLantern-A AND T-SpyTag
(Plasmid
#243776)
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PurposeExpresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 243776 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET 29b (+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemGreenLantern
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SpeciesSynthetic
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Tags
/ Fusion Proteins
- 6x His tag (C terminal on insert)
- SsrA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MGSS-CfaC-GGS(x3)-2A9c-GGS(x3)-mGreenLantern-GGS(x3)-TEVc-GGS(x3)-SpyTag003-GGS(x3)-6xHis-GGS(x3)-CfaN-SsrA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mGreenLantern-A AND T-SpyTag was a gift from Cole DeForest (Addgene plasmid # 243776 ; http://n2t.net/addgene:243776 ; RRID:Addgene_243776) -
For your References section:
Boolean logic-gated protein presentation through autonomously compiled molecular topology. Gharios R, Ross ML, Li A, Kottantharayil SP, Hoye J, DeForest CA. Nat Chem Biol. 2025 Oct 9. doi: 10.1038/s41589-025-02037-5. 10.1038/s41589-025-02037-5 PubMed 41068299