pI-SceI
(Plasmid
#243802)
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PurposeContains the ORF for the homing endonuclease I-SceI under the control of the strong, constitutive yeast promoter PTDH3.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 243802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepYTK001
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Backbone manufacturerJohn Dueber (Addgene #65108)
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pI-SceI was a gift from Sai Reddy (Addgene plasmid # 243802 ; http://n2t.net/addgene:243802 ; RRID:Addgene_243802)